Example 1: Testing a variegated leaf for starch
Adapted from June 2023 (R), Paper 1B · Question 6(b)(ii)
Question 1
Variegated leaves have areas that are green and areas that are white.
A student uses this method to investigate the effect of light on photosynthesis in a variegated leaf.
- place a plant in the dark for 24 hours
- wrap a strip of black paper across a leaf
- shine light on the plant for 24 hours
- remove the black paper
- use iodine solution to test the leaf for starch
The diagram shows the apparatus the student uses.
Diagram 1 shows the position of the black paper on the leaf.
Diagram 1
Complete diagram 2 to show where the variegated leaf would appear black after testing with iodine solution.
Diagram 2
Final answer
Shade both green areas exposed to light, above and below the black paper. Leave the white areas and the whole strip previously covered by black paper unshaded.
Mark scheme points
- M1 Shade both exposed green areas, leaving the areas with no green unshaded.
- M2 Leave the entire strip under the black paper unshaded. This mark is not awarded if the rest of the leaf is left blank.
Clear labels such as “black”, “starch” or “no starch” are accepted instead of shading.
Explanation
Iodine turns blue-black where starch is present. The initial period in darkness removes stored starch, so this test shows where starch has been made during the investigation.
- The exposed green areas contain chlorophyll and receive light, so they photosynthesise and produce sugars that can be stored as starch. Shade both of these areas. The white areas lack chlorophyll, so leave them unshaded.
- The black paper blocks light. Even the green parts beneath it cannot photosynthesise, so leave the whole covered strip unshaded.
Common mistakes
- Some candidates shaded green areas beneath the paper. Check that every shaded area had both chlorophyll and light.
- Leaving the whole leaf blank does not earn the mark for an unshaded covered strip: you must also show where starch is present.